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HPLC Basic Course

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We inform of Basic of HPLC from "Principle and System Configuration of HPLC" to "Merits of Various Detection Methods".

1. Principle and System Configuration of HPLC (1)

Principle of chromatography

2. Principle and System Configuration of HPLC (2)

Philosophy of Qualitative analysis and Quantitative analysis by chromatography

Qualitative analysis
Quantitative analysis

3. Principle and System Configuration of HPLC (3)

Configuration of an HPLC system

Pumping unit
Sample-injection unit
Separation unit
Detection unit
Data-processing unit

4. Separation Mode and Column (1)

Type of separation mode - Separation depends on the triangle relationships among the sample, filler, and eluate!

Adsorption mode
Partition mode

5. Separation Mode and Column (2)

Type of separation mode - Separation depends on the triangle relationships among the sample, filler, and eluate! (2)

Ion exchange mode
Size exclusion mode
HILIC mode

6. Separation Mode and Column (3)

Selection of eluate

7. Merits of Various Detection Methods (1)

UV/UV-VIS detectors

Diode array detector (DAD, PDA: Photodiode Array Detector)

8. Merits of Various Detection Methods (2)

Fluorescence (FL) detector

Differential refractive index (RI) detector

Conductivity detector

Electrochemical detector (ECD)

Evaporative light scattering detector (ELSD)

Corona® Charged Aerosol Detector® (Corona® CAD®)

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