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  5. UV-Visible/NIR Spectrophotometers (UV-Vis/NIR)
  6. UV-Vis/NIR Spectrophotometer Basic Course

UV-Vis/NIR Spectrophotometer Basic Course

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We inform of Basic of Spectrophotometer from "What can an UV-Vis Spectrophotometer Do?" to "Mechanism of the Spectrophotometer".

1. What can an Ultraviolet and Visible Spectrophotometer Do?

Determine the concentration (contents) of a solution
Determine the characteristics of a material
Determine molecular structure

2. Colorimetric Analysis (1)

Light and color

The relationship between light, radio, and X-rays (about light)
Sunlight vs. the laser beam (white light and monochromatic light)
Why is an apple red? (about color)
Invisible light (ultraviolet and infrared)
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3. Colorimetric Analysis (2)

Neglecting cleaning increases absorbance (Bouger-Beer's law).

4. Colorimetric Analysis (3)

Measurement by color strength (quantitative analysis)

Sample and standard solution
Magic drug (coloring reagent)
Calibration curve
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5. Colorimetric Analysis (4)

See a color (spectrum)

What's a spectrum?
What can be determined from a spectrum?
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6. Structure of a spectrophotometer (1)

Differences between a man and a spectrophotometer (rough structure)

7. Structure of a spectrophotometer (2)

Optical system

Is there a dedicated position for a sample? (Single beam and double beam)
Careful selection of wavelength (single and double monochromes)

8. Structure of a spectrophotometer (3)

Components of spectrophotometer

Light source
Spectroscope
Sample cell
Detector

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