Auto Capture for Array Tomography ACAT

Automated Image Acquisition System for Serial Section Analysis

ACAT automatically adjusts, aligns, and captures a specific region of interest (ROI) across multiple serial sections. Images are automatically acquired by specifying a ROI from a low-magnification SEM image or specifying the intercept position from an optical image/overlay.

Samples Courtesy of:
Dr. Daisuke Koga,
Asahikawa Medical University
and
Dr. Satoshi Kusumi,
Kagoshima University

Features observation case Specifications

Features

Import and easily identify a region of interest from an optical image
Correlate optical data with low-magnification SEM images to target ROI with high precision
Simplify and automate imaging of subsequent sections by creating a unique indicator tag for the ROI using the first acquisition
Correct rotation changes as well as section irregularities (compression, tears, etc.) by using integrated image processing in order to reduce deviation in the field of view and achieve artifact mitigation with increased accuracy

Samples Courtesy of:
Dr. Daisuke Koga,
Asahikawa Medical University
and
Dr. Satoshi Kusumi,
Kagoshima University

This group of approximately 60 sections mounted on a glass slide were captured by SEM and superimposed on an optical image.
The position of each section and the specified ROI were registered and displayed on the screen,
whereafter the highresolution SEM images were automatically captured.

observation case

Number of shots: 117 shots
Slice pitch: 70 nm
3D Reconstruction Software: Image-Pro Premier 3D
(Media Cybernetics Inc.)

Samples Courtesy of : Dr. Daisuke Koga,
Asahikawa Medical University
Ultra-thin Sectioning: ARTOS manufactured
by Leica Microsystems

Specifications

Main Specifications

Number of specified sections	Up to 400 sheets
Number of shots in a single section	Up to 400 sheets
Captured image resolution	640 x 480, 1,280 x 960, 2,560 x 1,920, 5,120 x 3,640 pixels
Photographed image format	BMP, TIFF, JPEG